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[9003577.] رقم البحث : 9003577 - |
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Expression pattern of heat shock protein genes in sheep / |
| تخصص البحث : General |
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Mansoura Veterinary Medical Journal. / Vol 21 Issue 1 - March 2010 |
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تاريخ تقديم البحث 08/01/2020 |
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تاريخ قبول البحث 09/01/2020 |
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عدد صفحات البحث 15 |
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FAWZY ELESSAWY MOUSTAFA YOUNIS ( alphayounis@gmail.com - 00201018090085 ) - مؤلف رئيسي |
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HSP70, HSP90, Real time PCR, Cytokines, Sheep |
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Objective: To recognize the Expression patterns of HSP 70 and HSP 90 genes of two local sheep breeds.
Design: Descriptive study
Animals: Fifty ewes (25 Barki and 25 Abu Dlik).
Procedures: This investigation was carried out on fifty sheep at the northwest coast and southeast of triangle Halayeb and Shalateen during the months of March and May 2018 and 2019 (average day time temperatures: 25–35 ?C; relative humidity: 55–65%). Total RNA was extracted using easy-RED™ Total RNA Extraction Kit. The primers for qPCR were designed on the basis of prior sequence information available at National Center for Biotechnology Information (NCBI) for with the target HSP70 and 90 genes. The Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) gene, housekeeping gene, was used for normalization qPCR data. The desired genes were amplified for relative expression measurements. Tumor necrosis factor-? (TNF-?), Interleukin-1? (IL-1 ?, IL-6, IL-10, IL-12) were assayed.
Results: The expression levels of the HSP70 and HSP90 genes and the genes in Abu Dlik sheep were observed to relatively up-regulated than those in Barki sheep. Abu Dlik breed exhibited an up-regulate mRNA level of Hsp70 and Hsp90 genes (1.70440938 vs 1.362954) while the Barki breed showed a down-regulated pattern (0.8550442 vs 0.4289764). In Abu Dlik ewes HSP70 gene exhibited a higher mRNA level than HSP90 mRNA.
Conclusion: Gene expression patterns of HSP 70 and HSP 90, as well as cytokines modulations, can be used as a biological marker and a reference point in animals to identify, manipulate and cross-breed for improving the genetic potential and adaptability in sheep which tolerates the harsh environmental conditions, especially the heat stress., and it is necessary to manage stress at a cellular level. |
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[9003587.] رقم البحث : 9003587 - |
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The effects of orally supplemented ketoconazole and griseofulvin on the fertility of male rabbits / Adverse effects of some antifungal agents on fertility in rabbit |
| تخصص البحث : |
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Mansoura Veterinary Medical Journal. / Vol 21 Issue 1 - March 2010 |
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تاريخ تقديم البحث 13/01/2020 |
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تاريخ قبول البحث 13/01/2020 |
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عدد صفحات البحث 23 |
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Amal Mokhtar Mohamed ( amal2325764@yahoo.com - 01208652860 ) - مؤلف رئيسي |
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ketoconazole,Griseofulvin,Fertility,Rabbit |
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Objectives: The present study was designed to investigate the potential side effect of ketoconazole and griseofulvin on fertility of clinically healthy male rabbits.
Design: Randomized controlled experimental study.
Animals: Thirty mature male rabbits.
Procedure: Rabbits were randomly allocated into three equal sized groups (10 animals each).The first group was served as control, without treatment while 2nd group received ketoconazole (50 mg/kg) orally for 30 days . The 3rd group received griseofulvin (25 mg/kg) orally for 30 days. Semen sample were collected after 1 day, 1 week and 2 weeks post-dosing for determination of sperm motility, % of live and dead sperms, total of sperm abnormalities and sperm cell concentration. After 2 weeks post dosing testes and epididymisepididymes were removed for histopathological examination. |
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[9003590.] رقم البحث : 9003590 - |
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Efficacy of clove and thyme against experimentally induced Candidiasis in broilers / Antifungal effect of clove and thyme against Candidiasis on broilers |
| تخصص البحث : |
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Mansoura Veterinary Medical Journal. / Vol 21 Issue 1 - March 2010 |
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تاريخ تقديم البحث 13/01/2020 |
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تاريخ قبول البحث 13/01/2020 |
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عدد صفحات البحث 28 |
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ghada abdelfatah hassan abouzead ( agdyj5f50@yahoo.com - 01092953888 ) - مؤلف رئيسي |
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: clove powder, thyme oil, candida, chicken |
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Objectives:The present study was designed to investigate the efficacy of clove powder and thyme oil against experimentally induced candidiasis in broiler chicken.
Design:Randomized controlled experimental study.
Animals: One hundred and twenty five chicken (one month old) were used in this study
Procedure:Chicken were randomly allocated into 5 equal sized groups (25 each).The 1st group was used as negative control. However,the other groups were experimentally infected with candida (C.) albicans. The 2nd group was served as positive control without treatment. Meanwhile, the 3rd,4th and 5th groups received clove powder (2%), thyme oil (200ml/kg) in diet and fluconazole (5mg/kg) for10 days, respectivelyafter the appearance of symptoms which include delayed gastrointestinal transit times, static crop or sour crop. Anorexia and regurgitation. The normal thin wall of the ingulvies become thickened with complete crop stasis.
Results: clove powder and thyme oil showedimprovement in the recorded symptoms induced by C.albicansthey also significantly increased PCV%, ALTandASTlevelwithadecrease in erythrocyte count and total leukocytecount. Clove powder increase in creatininelevel,whilethyme oil decrease creatininevalue.The tested plants induced marked histopathological alteration in crop, lung, intestine and gizzard of treated birds. |
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