الفهرس 
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Abstract
In this study, 120 freshwater fish (Oreochromis niloticus) were divided into 4
different groups, each in triplicate groups (n=10), and used to assess the
pharmacological activity of ZnO-NPs as anti-bacterial, immunomodulatory and antioxidant agent. Initially, the antibacterial activity was detected in-vitro through the
disk diffusion method. The results revealed a potent antibacterial activity, especially
at 80 mg/kg of ZnO-NPs. Then, Pathogenicity test with pseudomonas aeruginosa
was done. The clinical signs following the infection were also detected and the
mortality rate was calculated. Administration of ZnO-NPs (80mg/kg) showed potent
ability to protect the fish from mortality induced by I/P injection of P. aeruginosa.
Blood samples were collected twice, on the 20th and 40th days to detect the effect of
ZnO-NPs on some immune parameters including granulocyte, monocytes,
lymphocytes and white blood cells (WBCs) count, immunoglobulin M and
lysozyme. Finally, tissues for histopathology to detect the effect of ZnO-NPs on
some key immune organs The oral administration of Zn-ONPs resulted in a
significant increase in Albumin, globulin, total protein, lymphocyte, monocyte,
immunoglobulin M , lysozyme activity and glutathione from the 20th to 40th days in
infected tilapia. In contrast, the results of ALT, AST and MDA decreased from 20th
to 40th days in groups exposed to 80mg/kg ZnO -NPs. In conclusion, ZnO-NPs
showed significant antibacterial activity against P. aeruginosainfected tilapia fish
either in-vitro or in-vivo as well as elevated the immunity of the fish against P.
aeruginosa infection.