الفهرس 
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Abstract
Multi drug resistant bacteria have become one of the most important causes of serious opportunistic diseases worldwide. Bacteria are one of the main pathogens of most diseases and epidemics in water, air, or hospital environments. This study aims to isolate and characterize Multi drug resistant bacteria and to synthesize nanoparticles. Our letter includes the cytotoxicity of silver particles on human cell lines.
Nowadays, bacterial resistance to different categories of antibiotic has been developed. Antibiotic resistance is one of the key threats of global health. Here, tow isolates from B Al-Zahraa hospital faculty of Medicine (Girls) are identified biochemically and morphologically and confirmed also using the automated Vitek 2 system.
Antibiotic sensitivity pattern of the isolated bacteria was assessed against selection of twenty-three antibiotics, the isolates of Klebsiella pneumonia showed resistance against 95% of tested antibiotics and E. coli show 83% resistance from different categories which make them followed Multi drug resistant bacteria (MDR).
This study aimes was designed to Synthesis of Nanoparticles from pomegranate peel extract against certain antibiotic-resistant bacteria. To overcome this problem
The results showed significant inhibition with promising antibacterial parameters, based on the preliminary antibacterial results, the MIC and MBC were measured toward and the experiments were carried out three times to confirm the MIC and MBC data. The MIC assay for the PP extracts was found about 32 µg/ml for Klebsiella pneumoniae . while the MBC was 64 µg/ml. and The MIC assay for the PP extracts was found about 34 µg/ml for Escherichia coli strain. while the MBC was 64 µg/ml. The results revealed that the extract exhibited
The highest antibacterial activity was recorded for AgNPs The MIC assay for the AgNPs was found about 4µg/ml for Klebsiella pneumoniae . while the MBC was 8 µg/ml. and The MIC assay for the AgNPs extracts was found about 8 µg/ml for Escherichia coli strain. while the MBC was 8 µg/ml. The results revealed that the extract exhibited bactericidal activity against the tested strains. Klebsiella pneumoniae and Escherichia coli was killed by 2-AgNPs within 4 hours for Klebsiella pneumoniae and 3.5 for Escherichia coli.
AgNPs have a zeta average of d.nm 131.7; and zeta potential (-14.8mV); which confirmed by TEM.
Two MDR strains were identified through 16S rDNA; their nucleotide sequences were compared against Genebank database through BLASTn which showed that isolate identified as Klebsiella pneumoniae and E. coli, isolate They have accession numbers OP896863, OP143701, OP863037.
The cytotoxic effects of the powdered extract of pomegranate peel and AgNPs were also tested against a (HepG2: Hepatocellular carcinoma cell line; HeLa cell line; MDA-MB-231: Breast cancer cell line; WI-38:), and it was observed that the viabilities were 56% and 61% 48 hours respectively, with exposure to different concentration of AgNPs. This simple, economic, and eco-friendly method suggests that the AgNPs biosynthesized using pomegranate peel extract may be a novel, potent solution for the development of a drug for cancer cell line.
Antioxidant activity of AgNPs was conducted using several methods (DPPH, Fe3+-Reduction capability, Fe 2+ ion chelation ability, Lipid peroxidation Inhibition capacity, O2- radicals scavenging capacity, NO scavenging capacity)