الفهرس Only 14 pages are availabe for public view
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Abstract
BMI-1 and SALL4 are stem cell genes that modulate stem cell pluripotency so they may play a role in leukemogenesis. The aim of this study was to characteriseSALL4 and BMI-1 gene expression patterns myeloid leukemia and in patients who have achieved complete remission after chemotherapy.Real time PCR was used to assess the gene expressions patterns in 106 patients;54 AML patients (43 de novo AML, 11 AML in CR) and 52 CML patients (31 in chronic phase, 11 in deep molecular response (MR4) and 10 patients in accelerated and/or blastic phase), in addition to 21 non malignant bone marrow samples. The study revealed an increased expression of SALL4 gene in AML de novo, AML-CR compared to the control without significance difference between both groups. CML-CP patients showed increased expression of SALL4 and this expression lowered in CML-MR4 and CML-AP/BP (median= 14.125, 0.592 and 0.593 respectively, p=0.064).CML-CP patients showed a significantly higher percentage of patients with high SALL4 expression as compared to both CML-MR4 and CML-AP/BP (p=0.033). BMI-1 gene expression was not found to be increased in any of the patient groups. These results describe the altered SALL4 gene expression pattern in different phases of myeloid leukemia. The role of BMI-1 in myeloid pathogenesis needs furtherdelineation to determine its significance