الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
Rift Valley Fever (RVF) is an acute febrile, contagious, .pod born, zoonotic disease caused by Bunyaviridae of the genus phlebovirus. It causes high rates of ion and neonatal mortality in sheep and cattle. :ptibility to infection and to disease decreased with age. 1 mortality rate, reaching 100 may occur in lambs less one week old. Animals other than sheep and cattle are eptible to a much lesser extent. I Therefore, this study was aiming to detect the serum ibodies of Rift Valley Fever virus among the domestic mals and personnel, whose work are associated with ~m, and the possibility to identify post infection from post ccination antibodies by rbent assay (ELISA) using enzyme linked immuno and immunodiffusion (ID) chniques. Also, this study was aiming to detect Rift Valley ever viral antigen in Culex pipiens mosquitoes collected rom study areas by . uSIng double (sandwich) ELISA echnique. A total of 300 human blood samples, 300 sheep blood samples, 311 cattle blood samples and 100 pools of female Culex pipiens were I . collected from 3 different governorate; Alexandria governorate which represented free epizootic area of Rift Valley Fever (up to 152. of the collection of samples); Minia govemorate which :d the area of reported epizootics and is representative of ;ypt and Sharkia govemorates which represented the area t evidence of epizootic situation of Rift Valley Fever. iSA technique for detection of antibodies against Rift ever virus was described by Swanepoel et al (1986a) and by n et al (1984a). The ID technique was described by Ayoub till (1981), while ELISA test for detection of Rift Valley ral antigen was described by Niklasson and Gargan (1985). he results of this study revealed that: of 300 human blood samples,