الفهرس 
Contents
morphologyOnly 14 pages are availabe for public view
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Abstract
Hpylori is one of the most common pathogens of humans worldwide. .. It inhabits the gastric mucosa for decades and it is more prevalent in developing countries and among those living in low socioeconomic conditions. Infection mostly occurs in young age and unless treated, remams active for life. . The organism is now accepted as the main cause of active type B chronic gastritis and a major etiologic factor in peptic ulcer disease; it is also implicated in the pathogenesis of gastric carcinoma and type B cell lymphoma. - The interplay between bacterial, host and environmental factors are important in defining the clinical outcome of infection. - Diagnosis and treatment of this infection has become necessary for achieving long lasting cure of the resulting pathologies. It includes: InVaSIVe methods depending on biopsy taking through upper gastrointestinal endoscopy and non invasive methods as urea breath test and serology. Because of the difficulty in recovering this fastidious organism from biopsy material recent attention has focused 0’1 immunologic diagnostic techniques. . 129. ” . This work was carried out to evaluate the accuracy of a newly introduced serological test for the rapid diagnosis of Hpylori infection in dyspeptic patients. . 100 dyspeptic patients presenting for endoscopy were enrolled in this study. . Their ages ranged from 14 y to 68 years (mean age == 40y) . There were 65 males and 35 females. . Each patient was subjected to a full history taking, clinical and \’I endoscopic examination. . From each patient we obtained: 1 - 3 antral biopsy specimen to perform rapid urease test, culture and direct Gram films. 2 - 3 ml blood sample, collected by venipuncture prior to endoscopy, in order to detect anti H.pylori antibodies using the ”H. pylori Stat-Pak” kit. - Two antral biopsies were immediately inserted into the agar gel of ”hp fast” rapid urease test performed in the endoscopy room. ~ One biopsy was transported to the microbiology lab in a sterile tube containing 0.5 ml saline and was processed within one hour: . The biopsy was slightly crushed using a sterile glass rod, then inoculated by rubbing over the surface of a freshly prepared plate of selective Colombia agar supplemented with 7-10 whole sheep la T 130. ” blood and H.pylori selective supplement containing antibiotics. I I I Plates were then incubated in a microaerobic atmosphere, using a Campy gas pack with a catalyst, at 37°C for 3-5 days. i~ Suspected colonies were subjected to Gram stain and biochemical tests (urease, catalse, oxidase) for identification. . Multiple colonies were then swabed and inoculated into either Trypticase soy broth or Brain heart infusion broth to produce a cloudy suspension; a swab was dipped inside, squeezed out and immediately streaked over the surface of a Columbia blood agar plate to perform an antibiotic susceptibility testing. Antibiotic disks were distributed and plates were incubated at 37°C in a microaerobic atmosphere for 4-5 days and diameters of the zone of growth inhibition was measured for each antibiotic disk. .