الفهرس | Only 14 pages are availabe for public view |
Abstract Composting depends on biological process to decompose organic compounds of solid waste. Composting of rice straw or garbage supplemented with chemical accelerator, sheep manure and mixture of cellulolytic microorganisms was conducated and mixture of Azotobacter chroococcum and P-solubilizing bacteria as biofertilizer was added to half amount of compost to give qualified one. The enhanced use of bioinoculum in combination with chemical amendments accelerated the compost maturity and shorted the usual period of composting. Cellulolytic bacteria and fungi isolates were isolated from different sources. The isolates which gave faster positive results for their cellulase activity were identified as Micrococcus luteus , Kocuria rosea and Stachybortys sp. These strains were subjected to further environmental and nutritional requirements. The optimum conditions for maximizing enzyme activity was addition of CMC as the most active carbon source for growth proliferation and enzyme activity for all cellulolytic strains at 1% concentration. Also, addition of yeast as nitrogen source at 0.8 g/L for bacterial strain ; MnSO4 (0.5 g/L) for Micrococcus luteus and CaC12 0.5 g/1 for K. rosea as metallic ions and incubation period at 45°C and pH 7.0.for 1 day and 4 days, respectively. For Stachybotrys sp., addition of peptone 2.2 g/L as N- source and CoSO4 (0.5 g/L) as metallic ions and incubation period for 5 days at 30°C and pH 5.5 gave the maximum enzyme activity. |