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Abstract 1- The most common fungi isolated from Zagazig area (cultivated land ) were identified as follow:- Aspergillus niger,Aspergillus flavus, Aspergillus fumigatus and Fusarium sp.. Rhizoctonia solani was isolated from infected roots of cotton seedlings from the same area. 2-The optimum temperature for growth, of all tested fungi, was 30 °C. Change of nitrogen source, in nutritive medium, had no effects on the optimal temperature for growth. 3-The lowest growth, obtained at the highest incubation temperature (35 °C,) in the case of Rhizoctonia solani, Aspergillus fumigatus and Fusarium sp., and at the lowest incubation temperatue (20 °C) in the case of Aspergillus niger and Aspergillus flavus. 4-The presence of cyolane in the nutrient media revealed a suppersive effects on the growth of all tested fungi, especially at higher concentrations (300,400 and 500 p.p-m). -132 - 8- The optimum pH for growth in absence of cyolane (control), at the optimum incubation temperature 30 °C, ranged from 5 for Aspergillus flavus to 6 for Hhizo-ctonia solani in presence of any type of nitrogen. On the other hand the optimum pH for growth of Aspergillus niger, Aspergillus fumigatus and Fusarium sp. was 6 in presence of sodium nitrate in culture medium and 5 in presence of ammonium sulphate. 9-The higher concentration of cyolane (300,400,500 p.p.m) in presence of sodium nitrate as nitrogen source shifted the optimal growth of Rhizoctonia solani to the alkaline side from 6 to 7, however the same concentra-tions of cyolane shifted the optimal pH for the growth of Aspergillus niger to the acidic side from 6 to 5. 10-The presence of cyolane in culture medium in presence of any nitrogen source had no significant change in protein - N content of mat for all tested fungi. 11-The polysaccharide content of mats (in mg/gram dry weight ) for all tested fungi, gradually decreased by increasing the concentration of cyolane in untritive medium from 200 up to 500 p.p.m, in presence of any type of nitrogen. -- 133 - 12- The addition of cyolane to the culture medium revealed significant reduction on total lipids of mats for all tested fungi. |