الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
The most important intestinal infections caused by Enterobacteria are the enteric fever, salmonella food poisoning and gastro-enteritis caused by shigella, y. parahaemolyticus and enteropathogenic E.coli. Enteric diseases are closely related to the environmental conditions and the sanitary facilities, because these organism ca~ be transmitted by contaminated water with excreta&of man or animals and such water may then be of public health hazard. ’~ The isolatio~of the salmonella shigella and v. parahaemolyticus directly from water is the most reliable proof that it contains dangerous impurities. The present i , study aimed at isolating, identifying and typing of some pathogenic organisms as salmonella, shigella, and v. parahaemolyticus from different parts of Mariut l~ke, and to evaluate the health hazards resulting from this lake. To achieve this goal the following procedures were carried out: A total of 80 water samples were collected by the modified Moore swab from. four different sites (Factories, Gheit El-Enab, .Kabbary, Qalaa) which are known to receive j j the sewearage draining from previous sites. These swabs were tran~ferred to: bottles of S.F. broth as enrichment for salmonella, shigella; GN broth as enrichment for shigella and alkaline peptone water was used as enrichment broth for !.parahaemol:rticus; all the bottles were incubated for 18-24 brs. at 37°C except S.F. broth ’to which was incubated at 4l.5~C. All the incubated media were inoculated on solid media: BS, Mc, XLD agar for salmonella and sh:1gella (BS’agar for salmonella’only), and TCBS plates for y. parahaemolyticus. All the plates were incubated at 37°0 for 24 hrs, except BS agar which was left for 48 hrs. ., Suspected colonies were identified biochemically and serologically according to Edwards and Ewing methods. Out of the 80 examined samples, 56 (70) were positive for salmonella, 4 (5) were positive for shigella and there was no !.parahaemolyticus recovered. In this study it was found that the highest salmonella percentage (90) were obtained from Gheit El Enab, Kabbary and Qalaa, while only 10 were obtained from factories site. from this study, 212 salmonella strains were isolated. They were belonging to 20 serotypes. Out of them, 3 (1.5) strains (from 20 samples) from ~actories site, 71 (33.5) ~om Gheit El Enab, 56 (26.4) from Kabbary, and 82(38.7) from Qalaa. The predominant serotypes were S.paratyphi B 102 (48.1)~ S.agona zO (9.5), S. montevideo 14 (6.6), S.wien 12 (5.7), and S.typhimurium 8 (3.8). In addi tioD 4 (5) shigella strains, they were isolated ~.boYdii 2 and .Sh. boyd1i 3 serotypes isolated from Gheit El-Enab, and Kabbary 3 (75) I (25) respectively. It was evident that Bs agar was the most efficient medium. Since it yielded 138 (65.1) salmonella isolates especially when it w~ preceded by S.F. broth. from the results of this study, the swab method seems to be the method of choice for qualitative determi nation of enteric pathogenic organisms.