الفهرس 
Tuberculosis in EgyptOnly 14 pages are availabe for public view
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Abstract
Tuberculosis describes an infectious disease that has plagued humans since the ancient times. It has been affecting man for at least 5.000 years. (Stark, 2000). The emergence of anti-tuberculous drug resistance, especially multidrug resistant tuberculosis (MDR-TB), poses a serious threat to the success of TB control programs. [Suresh et al., 2006].This study aimed to evaluate peripheral blood based PCR technique in the diagnosis of active pulmonary tuberculosis . It also aimed to use manual DNA sequencing technique for sequencing 3 selected genes. To achieve this goal, 40 patients were selected from those who were diagnosed to have pulmonary tuberculosis by their history ,clinical examination , radiological finding and laboratory criteria. The main laboratory criteria for selection was their +ve smear stained by Z.N for sputum samples showing AFB. All patients had history of treatment failure with RIF and INH for at least 6 months. 20 healthy blood donors were chosen as a control group. PCR results showed 100% specificity and 100% sensitivity. 15 samples from those which were PCR +ve for IS6110 gene were randomly selected where manual DNA sequencing was done for 3 selected genes : rpoB , katG and inh-A gene. Our results revealed that mutant rpoB genes were 93% versus 7% which were non mutant. The sequenced katG genes showed 100% point 87% of manually sequenced inh-A genes were mutant versus only 13% which were non mutant.