الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Visceral leishmaniasis is caused by protozoal parasi tes the genus Leishmania. They are originally parasites of rodents and have adapted to canin~ and man. The parasi te between two hosts, the vertebrate hosts (man, rodents), .and the invertebrate host (sandflies). the vertebrate host it is present as amastigote which is and lives in phagocytic cells. It is present as promastigotes in the gut of the invertebrate The surest method for diagnosis of visceral leishmaniasis the demonstration of amastigotes in the materials aspirated from bone marrow, spleen, liver or lymph nodes. However, several immunological tests were introduced to make the dia sis more easy for field application. The present study was carried out in order to evaluate three immunological tests, viz, leishmanin skin test (LST), irect immunofluorescent antibody test (IFAT) and indirect lagglutination test (IHAT), in the diagnosis of L. donovani ection in mice. donovani promast i g otes (Sudane se s t r a in) were grown on ’s and El-On t s media, to prepare LST reagent, IFAT anti and to infect mice. H.I.P.H.) were inoculated with L.dono promastigotes. mice were inoculated using intraperitoneal e with 2X107 promastigotes/mouse. These mice were followed r 28 days. ii1’h e in t r a car d i a c r 0 u t e was use d t 0 i n 0 c u 1 ate 3 groups -Group I :40 mice, inoculated with lXl07 promastigotes/ mouse. -Grou p II :42 mice, inoculated with 2Xl07 promastigotes/ mouse. - -Group III :25 mice, inoculated with 4Xl07 promastigotes/ All mice inoculated with the intracardiac route were wed up for 3 months except group III which was followed r 2 months. mice was done 24 hours, 7 days, 14 days, and 3 months post-inoculation. Five mice ~ 11 and 4 mice from group 111 were sacrificed Sacrifaction was done for: the liver and spleen parasite loads. liver and spleen weights. -Calculating li ver and spleen/body wei gh t rat i 0 s . -Collecting blood DROPs on fil ter papers for performance of IFAT and IHA T. The le~hmanin skin test was performed 24 hours before :Htion to be read on the next day. Ana 1 Y s is of the res u 1 t s rev e ale d t hat: mouse strain of the H.LP.H. was found to be initially iceptible to Leishmania don9~ani infection. This suscep lity was less than that of the highly suscepti ble mice le mice, but slightly higher than that of the non eptible mice Mus musculus. susceptibility of the mouse strain of the H.LP.H. was d to be route and dose -dependent. The intracardiac e was effective, while the intraperitoneal route failed roducing visceral leishmaniasis. Inoculation of high 8 of promastigotes were more efficient in. infecting the inoculated mice using the intraperitoneal route did not Ij positive LST reactions during the whole follow up Ilad (28 days). inoculated mice using the intracardiac route, started LST from the 7th day post inoculation rd. Positive reactions increased proportionally with of infection and the parasite load of liver spleen. Inr started to detect antileishmanial antibodies fro~ first day post-inoculation in 14