الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Free Radicals have a significant role in the pathogenesis of cancer as the
accumulation of free radicals (FRs) leads to oxidative stress which is the risk
factor for the development of cancer as it can initiate lipid peroxidation, oxidize
proteins and cause D.N.A damage.
P53 is a tumour suppressor gene and the P53 mutation is the most common
genetic mutation in cancer. Loss of P53 function or inactivation due to nay cause
leads to decrease the ability of the cells to arrest cell growth at the G1/S check
point in response to DNA damage.
Altered P53 was associated with high stage/grade and poor prognosis.
Also, it is suggested to be predictor of decreased survival.
In our Study we collected (50) male subjects . Their age ranged from 38-80
years with the mean value 56.3 .All subjects included in this study were
categorized into the following groups:-
Group I : Included 10 healthy subjects served as a control group
Group II : Included 10 patients with grade I cancer bladder
Group III : Included 10 patients with grade II cancer bladder
Group IV : Included 10 patients with grade III cancer bladder
Group V : Included 10 patients with grade IV cancer bladder
All subjects were submitted for routine investigation and cystoscope
guided piobsy for histopathological grading. Then they were subjected for
determination of lipid peroxide (MDA) and P53 (in both tissue and serum)
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Our results showed that; serum LP in all grades of cancer was significantly
increased in both pre-operative and post-operative patients compared with the
control group (P<0.05) except patients of grade 3 who showed non- significant
Increased of post operative serum LP compared with the control group.
However, post-operative serum LP was significantly decreased compact with
pre-operative patients (P1<0.05) (tables 14, 15,16,17,18)(Fig 8,12,16,20,24)
On the other hand MDA in malignant tissue homogenates was significantly
decreased in grades III & IV of cancer compared with those obtained from
nonmalignant tissues but insignificant in GI&II (P<0.05) (tables 14, 15,16,17,18)
(Fig 10,14,18,22,26)
Additionally, serum P53 in all grades of cancer was significantly increased
in pre-operative and post-operative patients compared with the control group
(P<0.05). However, post-operative serum P53 was significantly decreased in
compared with the pre-operative patients (P1<0.05) (tables 14, 15,16,17,18)(Fig
9,13,17,21,25).
On the other hand, P53 protein in malignant tissue homogenates was
significantly increased in all grades of cancer compared with those obtained from
non-malignant tissues (P<0.05). (Tables14, 15,16,17,18).
Correlation study revealed that serum MDA showed a significant positive
correlation with serum P53 Protein in grade I (r=0.652 P < 0.05) grade II
(r=0.911 P< 0.05), grade III (r= 0.847 P< 0.05) and grade IV (r=0.641 P<0.05)
(table 19) (fig 28,29,30,31). Furthermore, serum MDA showed non- significant
negative correlation with tissue P53 in grade I,II and IV while non-significantly
positive correlated in grade III, (table 19).
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Moreover, tissue MDA was non significantly positive correlated with P53
protein in tissue homogenates in all grades of cancer except grade IV which
showed a significant positive correlation (r=0.826 P< 0.05) (table 20) (fig 32).
Moreover tissue MDA showed non-significant positive correlation with serum
P53 is grade I & IV while non-significant negative crenellation in grade II & III
table (20).
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Conclusion
We concluded that, an intimate relationship was found between
free radicals, lipid peroxidation and over expression of P53. However,
oxy-radicals cause genetic changes of P53 resulting in loss of
programmed cell death (appoptosis) which play a critical role in
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tumorigenesis. So, P53 was a useful prognostic marker for follow up of
patients with cancer bladder.