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Abstract
Internal exposure to chemical compounds is best measured by determining the concentration of the toxicant or its ultimate metabolite at the critical site in the target organ or by determining adducts with cellular macromolecules such as proteins, amino acids, DNA, or its bases. To evaluate changes in plasma proteins and RAPD profiles that reflect gene expressions and DNA effects were compared to fitness parameters in Callus gallus domesticus. Exposure to progesterone hormone concentrations via two different metabolic pathways; Img/chicks subcutaneous and l.Smg/ chick orally has been occurred. Numerous studies have shown that gene expression correlates with a change in DNA structure, which in turn may induce changes in RAPD profiles. Although the most common use of the SDS-Polyacrylamide gel electrophoresis and RAPD assays are related to genetic mapping, taxonomic and phylogenetic studies. The aim of the present study was to evaluate the potential of the SDS-PAGE and RAPD assays for the detection of different protein adducts and DNA alterations. Results revealed relatively a great genetic differentiation among the progesterone treated and control chicken groups considering the hormonal-effects of the analysed accessions. The effect of deletion of some protein fractions caused by the hormonal treatment may , be considered as a successful distinguished fingerprint of progesterone disruption after 3 j weeks of exposure.