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Abstract
Bone marrow transplantation is a standard line of therapy in many haematopoietic and nonhaematopoietic malignancies.In Egypt PBSCs has almost replaced the bone marrow as a source of graft in allogeneic BMT.This work was designed to study the different graft components and its possible impact on the development of GVHD.Fifty-one patients receiving allogeneic PBSC transplantation performed by the team at Nasser and National Cancer Institutes Cairo University, Egypt, in the period from Dec. 1998 to Aug. 1999 were included in this study. Their mean age was 25.0 years with male to female ratio 3,25:1. Their original diseases were as follows: CML (n=19), SAA (n=12), AMI (n= 10), ALL (n=3), HD (n=2) thalassaemia (n=2), MDS (n=1), MM (n=1)and NHL(n=1).
All donors were fully HLA- identical siblings except one (number 17 who
received a graft from his sister with one A locus dismatched).The dual color
analysis was performed using flow cytometry for CDS,CD4, CD8, CD16+56,
CD57, and CD69 positive cells. Th-1 and Th-2 cells were estimated by
measuring IFNy (for Th-1) and IL-4 (for Th-2) after activation with PMA to for
4 - 5 hours Follow up of patients was perforemed for at least 3 months for
detection of GVHD and other complications.