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Abstract Sewage water application in Arab Abou Saed region increased the concentration of Cu, Zn, Cd and Pb in the different organs of the cultivated bean, broad bean and tomato plants. Sewage water application stimulated seed germination of bean and growth of seedlings and mature plants. Sewage water increased the chloroplast pigments, total soluble sugars, total carbohydrate, nucleic acids, nitrogenous compounds and enzymes activities. Whilst free amino acids decreased, the protein content increased. Treatment of sewage water with precipitation, rice residue or EDTA decreased the leaf content of chloroplast pigments, carbohydrates, nitrogenous compounds, DNA and RNA, while the free amino acids content increased. Protein banding patterns of the produced seeds indicated that the appearance of new bands having high molecular weights was the marked feature in the sewage water irrigated plants. Precipitation, rice residue or EDTA treatments led to disappearance of some bands and appearance of others.The concentrations of Cu, Zn, Cd and Pb increased greatly in the different organs of bean in response to sewage water application to exceed the maximum permissible levels according to FAO. Treatment of sewage precipitation, rice residue accumulation. or water with EDTA decreased this |