الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Trichinella s p i r a l i s is a highly successful paras i t e i n t h a t it can e s t a b l i s h , developeand reproduce i n a wide range of vertebrate hosts, I n t e r e s t has been focused on the antigenic nature of Excretory-secretory (ES) antigens of larva of Trichj Della s p i r a l i s . I s o l a t i o n and characterization of ES antigens has been recently done, also, f r a c t i o n a t i o n of these antigens has occured. The antigenicity of each separate f r a c t i o n is not fully studied and t h e i r role i n e l i c i t i n g immunity against - T, s p i r a l i s are not well known. Thus t h i s study a i m a t preparation of ES antigens, t h e i r f r a c t i o n a t ion end studying t h e i r e f f e c t s a g a i n s t i n f e c t i o n with 2, s p i r a l i s , Five batches each of approximately I1 x 10 6 packed larvae were isolated from infected male albino r a t s . Komogenizstion of larvae, d i f f e r e n t i a l c e n t r i f - ugation of the homogemate, treatment with 0.1% Triton x -100 and ultracentrifugation yielded a soluble p a r t i c l e (large-particle fraction) designated S Biochemical 3’ characterization of S was found to be a glycoprotein, 3 Pull characterization of S was achieved by imunoele- 3 ctrophoresis against hyperimmune rabbit serum revealed - the presence of 20 antigens. Electrophoresis i n 10% SBS-polyacrylamide gel resolved a minimum of 28 proteins with molecular weights ranging from 11 t o 105 Kilodalton (Kd,). Further c h a r a c t e r i z a t i o n by molecular 5 s i z i n g column chrouatogrephy using Sephacryl S-300 yielded six peaks. Chenical c h a r a c t e r i z a t i o n of these peaks were found t o be glycoproteins with highest protein content i n the fourth peak. Immunoelectrophoresis shoved t h a t most of the antigens mere contained i n the t h i r d , fourth and f i f t h peaks and the highest number of these antigeos mere present i n the f o u r t h peak, Markers included in the chromatography system and SDS-Page indicated that these peaks contained materials with molecular weight of 100-150, 96-100, 64-95, 35-63, 15-34 and 11-14 Kd. f o r the first, second, t h i r d , fourth, f i f t h end the s i x t h peak respectively. Antigens were injected into adult male Swiss albino mlce together with an equaf. volume of Freund’s complete adjuvant, three i n j e c t i o n s one every week and one week l a t e r a l l mice were challenged with 200 muscle larvae orally. A control of buffer ( i n which the antigens were prepared) and non imunized infected group were done. The influence of immune response on mtce was determined by different procedures: 1) determination of T-lymphocytes present i n the spleen by E-resetting t e s t , 2) adult e x p u l a i ~ n frcm the sn:sll i n t e s t i n e , 3) qua l i t a t ive e s t i m s t i o n of imunoglob:~lin G (IgG) and immnoglobulin 14 (I,@) i n the s e r a of t e s t e d aninals by the double diffusicn (The Ouchterlony technique) and 4) numbers of larvae i n the muscles. Results showed t h a t irnramization with peaks other t h a n the f o ~ r t h peak 8.n.d S eLicited no m n i f e s t irimun- 3 olcg.’Lcal res;onse and t h e i r c r i t e r i a iz:sre tLe sane as that found i n mice i n x n i z e d with bliffer or co2trol group. On t3e o t h e r hen3 S and peak four have res~~lt- 3 e3 i n msked reslstsnce against i n f e c t i ~ n with - T, s p i r z l i s which was manifested on adult expulsiotl fron the in- - testine, elevsted T-lymphoc,ates i n the s ~ l e e n s of mice ahicb was nanifzsted i n day six post I n F e c t i o 2 pronotifig rapid expulsicz of a d u l t s fram i n t e s t i n e . Increase i n le=Jels of I& and IgI2 especLally IgIJ detected e a r l y tn the infection and SigriLf icant r e d ~ c t l o n of muscLe larvae at day 30 p o s t i n f e c t i o ~ ~ These results showed t h a t a soluble port ion of large particle f r a c t i o n from muscle larvae can be obtained and I t s main irnmnogenic p o d i o n can be separated by molecular sizing column cbronetography on Sephacryl S-300.