الفهرس 
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Abstract
The current study was conducted at the experimental station and tissue culture laboratory of Vegetable and Floriculture Department, Faculty of Agriculture, Mansoura University during the seasons of 2009 – 2011. A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub lemon verbena (Aloysia triphylla) by in vitro culture of shoot tips or nodal segments from mature plants. The results showed that the best method of surface sterilization is using NaOCl at 3 % for 10 minutes for nodal segments and 8 minutes for shoot tips. Nodal segments were found to be more efficient than shoot tips for lemon verbena shoots regeneration on MS medium (Murashig and Skoog, 1962) supplemented with BAP at 0.1 mg/L. Of the three different cytokinins ,6-benzyl aminopurine (BAP), kinetin (Kin) and thidiazrone (TDZ) evaluated as supplements to Murashige and Skoog medium (MS), BAP at concentration of 0.5 mg/L was found to be the most effective cytokinin in inducing multiple shoots. The frequency of shoot proliferation was markedly influenced by number of subcultures, since the highest percentage of shoot multiplication (100 %) as well as the large number of regenerated shoots/ explant (11.5 shoots) were obtained in the 4th subculture, after which there was a declined in shoot multiplication. Concerning rooting of the obtained shootlets , the best percentage of rooting (100 %) and the highest number of root formed per shootlet was recorded by using MS medium at half salt strength augmented with indole-3-butyric acid (IBA) at 0.5 mg/L and 20 g/L sucrose. Mixture of soil: sand: peatmoss (1: 1: 1) was the most suitable planting substrate for hardening and its use ensured high survival frequency (100 %) of regenerated plants prior to outdoor transfer.