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العنوان
Biotechnological studies on lavender plant /
المؤلف
kasem, Mahmoud makram.
هيئة الاعداد
باحث / محمود مكرم قاسم
مشرف / على منصور حمزه،
مشرف / اميمه محمد عبد الكافى
باحث / محمود مكرم قاسم
الموضوع
Ornamentals.
تاريخ النشر
2011.
عدد الصفحات
250 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
1/1/2011
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Department of Veg & Floriculture
الفهرس
Only 14 pages are availabe for public view

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Abstract

The current research was conducted at the Experimental Station and tissue culture Laboratory of the Vegetable and Floriculture Department, Faculty of Agriculture, Mansoura University during the seasons of 2008-2010. The main goal was performed to improve the in vitro propagation (micropropagation) of English lavender (Lavandula angustifolia) family Lamiaceae using two main methods of tissue culture technique. It included two parts, the first was the direct micropropagation method and the second was the indirect micropropagation method of this medicinally and aromatic plant. • This study revealed the following results : Firstly: for the direct micropropagation methods 1- The best explant type was the nodal explant. 2- Increasing multiplication was through using full strength medium supplemented with TDZ at 0.20 mg/L and sucrose at 30 g/L. 3- The tallest and the healthy roots were obtained from half strength medium fortified with IBA at 2.00 mg/L, while the highest rooting % and roots number were obtained by adding NAA to the nutrient medium. But, these roots get easily detached from the microshoots during transplantation comparing with IBA one. 4- Mixture of peat moss, vermiculite and loamy soil (1:1:1 v:v) was the more suitable for plantlets transplanting. secondly: for the direct micropropagation methods 1- The highest shoots number derived from callus was recorded for nodal explant cultured on medium fortified with 2,4-D at 0.75 mg/L and then subcultured on medium augmented with TDZ at 0.25 mg/L. 2- The tallest healthy shoots from nodal derived callus was by 3/4 strength medium supplemented with GA3 at 0.50 mg/L.