الفهرس 
Introduction and aim of workOnly 14 pages are availabe for public view
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Abstract
Fig mosaic virus (FMV) is a serious virus which infects fig crop in Egypt and other countries. FMV caused devastating losses by reducing either the yield and/or quality of figs fruits. Therefore, it was important to identify, characterize and isolate the FMV. Infected leaves of figs (Ficus carica L.) were collected from different seven locations in Alexandria Governorates during summer 2008. Biological, serological and molecular studies were conducted to identify and characterize the FMV. The symptomatology identification of FMV was recorded as: light chlorotic spotting, mottling, extensive chlorosis along the veins and leaf malformation. The use of electron microscope showed that long flexuous particles of 740 nm with a tail of 220 nm in purified virus sample. Electron micrographs of ultrathin sections of FMV-infected leaves appeared the presence of so DMBs. Also accumulation of starch grain and presence long elongated and flexuous virus-like particles surrounding the chloroplast, in parenchyma cell of FMV-infected leaves. In the I-ELISA analysis, the commercial general polyclonal antibody against Potyvirus, PVY give positive result with symptomatic leaves of fig plant. In DBIA infected plants give positive results with PVY antiserum; these results could be recommended FMV is potyvirus. Universal primers of potyviridae were successfully amplified 969 bp of NIb gene of FMV. This sequence was deposited in GenBank under accession number (Acc# GQ871933). By using degenerate primer amplification product of 374 bp has been obtained. The sequence was deposited in GenBank under accession number (Acc# Q288368). The purified CP gene was cloned in PCR-TOPO vector, subcloned into pPROEX HTa expression vector and transformed into the competent E. coli strain DH5α. After IPTG induction, the charged clone clarified that the CP-like protein was expressed at the expected molecular weight (13.7 kDa) in SDS-PAGE (i.e. it is a positive sense RNA).