الفهرس 
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Abstract
In this study, 1. We collected blood and cord samples from 25 pregnant women infected with HCV. Blood and cord samples of 30 healthy pregnant women were included as a negative control. Blood and cerebrospinal fluid (CSF) samples from 25 meningitis patients infected with HCV (16 males and 9 females) were collected. In addition, blood and CSF samples from 30 meningitis patients without HCV infection (14 males and 16 females) were used as negative controls.Blood and urine samples were collected from 25 patients infected with HCV (18 males and 7 females). In addition, blood and urine samples from 30 healthy individuals (20 males and 10 females) were used as negative controls. 2. Our study is concerned with detection of HCV antigen in some body fluids, HCV antigen was identified at 27 kDa molecular weight in serum, cord, urine and CSF samples of patients infected with HCV using sodium dodecyl sulphate polyacylamide gel electrophoresis (SDS-PAGE) and immunoblotting technique. 3. 105 serum samples were collected (75 of patients infected with HCV and 30 non infected individuals as a negative control). All these sera samples were tested using ELISA technique as a sensitive and specific assay to detect HCV antigen. In conclusion, HCV antigen was identified in some body fluids (cord, CSF, urine and serum) at 27 kDa molecular weight by western blot technique. Also, HCV antigen was detected in serum samples of HCV infected patients using ELISA with high degrees of sensitivity (92%) and specificity (93.3%).