الفهرس 
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Abstract
Objectives :- Determine the antimicrobial activity of aqueous and methanolic extracts of some plants in addition to commercial oils on isolates of Klebsiella strains, investigate the synergistic effect of extracts as antimicrobial activity in association with antibiotic upon bacterial growth and the effect of cultivation medium on enzymatic production. Method & Results :- Bacterial strains were isolated from thirty samples from market milk on selective medium, only five isolates were chosen and identified as Klebsiella, these five isolates of Klebsiella spp. were tested against a total 3 classes of antibiotic (0.5 mg/ml) ampicillin, bacitracin and ciprofloxacin by using the well diffusion method. Obtained data showed that Klebsiella spp. were naturally resistant to ampicillin antibiotic. Antibacterial activity of the both aqueous and methanolic extracts in addition to commercial oils of the different plant against Klebsiella spp. determined in vitro by the agar well diffusion method. Obtained data showed that the methanolic extracts of Hibiscus sabdarrifa exhibited the highest antimicrobial activity against all the strains followed by Tamarindus indica and Punica granatum. The Cinnamomum zylanicum oil was found to have the highest activity against Klebsiella followed by Eugenia aromatica and Hentha virdis. The effect of cultivation medium on enzymatic production was determined. Most of the tested strains showed luxurious growth on α–glucan like starch or pullulan as carbon sources, data showed that there was no positive correlation between growth and both of pullulanase and α–amylase enzymes production. Conclusion :- The methanolic extracts of Hibiscus sabdarrifa showed the highest antimicrobial activity followed by Tamarindus indica and Punica granatum. The cinnamon oil showed the highest antimicrobial activity against Klebsiella strains. Data showed that Klebsiella spp. were naturally resistant to antibiotic ampicillin. No positive correlation determined between growth and both of pullulanase and α–amylase enzymes production.