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العنوان
Propagation of some ornamental plants by tissue culture technique /
المؤلف
Ahmed, Ahmed Wahba Sayed.
هيئة الاعداد
باحث / أحمد وهبه سيد أحمد
مشرف / محمود عبد المنعم خفاجه
مشرف / عبد الله أبو الخير
مشرف / فيصل محمد سعداوى
الموضوع
Mussaenda luteola. Clerodendron glabrum explants. cytokinins. kinetin.
تاريخ النشر
2009.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
01/01/2009
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Agric Botany Department
الفهرس
Only 14 pages are availabe for public view

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Abstract

This work was carried out in the Tissue Culture Laboratory of Zohriya Garden, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2007 to 2009. Applying clorox at 15%, value of survival percentage of both Mussaenda luteola and Clerodendron glabrum explants rose to the significantly highest level. The cytokinin type affected number of shoots and shoot length of two plants significantly. The significantly highest number of shoots was obtained by applying BAP, followed with a significant difference by those resulted when kinetin was used. On the contrary, the effect of these three cytokinins on shoot length was an inverse to those mentioned above.The effect of cytokinin type on the number of leaves was significant in case of Clerodendron glabrum only. However, in both plants the highest number of leaves belonged to shoots treated with BAP, compared to the other cytokinins. The cytokinin concentration had a significant effect on the number of shoots of both plants. In the absence of cytokinins, the significantly lowest number of shoots was produced. Cytokinin concentration exerted a significant influence on the number of leaves of both plants. However, this effect was negative in case of Mussaenda and positive in case of Clerodendron .The highest number of Mussaenda leaves was produced on the untreated control shoots. With Clerodendron glabrum applying cytokinins at 3 ppm resulted in the highest number of leaves. The response of the two plants was somewhat contradictory to each other. Plants treated with BAP at 3 ppm, while the lowest number belonged to Mussaenda luteola shoots treated with TDZ at 2 ppm and the Clerodendron glabrum shoots grown on the untreated control media. Concentrations of IBA used in the rooting stage did not have significant effects on the number of roots, root length and content of total chlorophyl of Mussaenda luteola plants at rooting stage.At the acclimatization stage, characters of plant height, root length, fresh weight of stem, fresh weight of roots and dry weight of roots responded similarly to the previous application of IBA during rooting stage. The significantly highest values were obtained with IBA at 1 ppm, while the significantly lowest ones were observed with IBA at 3 ppm.With characters like fresh weight of leaves, dry weight of leaves and dry weight of stem the significantly highest values were achieved as a result of using IBA at 1 or 2 ppm, while the significantly lowest records were a response to either the control or the 3 ppm treatments .