الفهرس 
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Abstract
absence of glycyrrhizin in both. This method provided an accuracy of 97.08 ± 2.08%, a recovery of 94.15 ± 2.13, and a lower detection limit of 50 ng.
Part 4: Preliminary Studies On The Production Of Callus Culture Of Glycyrrhiza glabra L.In this part, the production of a callus from the aerial parts and rhizome buds of Glycyrrhiza glabra was tried. This part began with a short introduction, involving previous work on the tissue culture of G. glabra. The practical work involved the choice of the proper conditions for initiation, propagation, and maintenance of the callus from the aerial parts of G. glabra.
The results indicated that the best conditions for the callus growth were as follows: the use of L.S. media, Kinetine (0.5 mg/L) as a cytokinine, 2,4 D (0.6 mg/L) as an auxin, the apical part of the shoot was used as an explant, the carbon source was sucrose (30g/L), the pH of the medium was 5.8, and the temperature was 25 °c. The growth begins in the dark, and then transferred to light after 3 weeks. The callus began to grow after 2 weeks, giving a bright yellow colour, which faded with subsequent sub-culturing.
Part 5: The Biological Activity
In this part, the anti-inflammatory, and the hepato-protective activities of the aerial part extract of Glycyrrhiza glabra was investigated.
The anti-inflammatory Activity was measured by the ability of the IP injected extract to reduce the carrgenin induce edema of the rat hind paw relative to the hydrocortisone (200mg/kg). The total extract at doses of 200 and 400 mg/kg, fractions of the total extract, light petroleum fractions (A, B, and C), chloroform (fractions D) and ethyl acetate fraction E) at doses of 400 mg/kg were tested.