الفهرس 
Subjects and methodsOnly 14 pages are availabe for public view
 |  | from | 135 |  |  |
| | | from | 135 | | |
Abstract
Thrombocytopenia is the most common cause of abnormal bleeding. Immune thrombocytopenia characterized by increased platelet destruction by antiplatelet antibodies. Despite an extensive search for a definitive assay for platelet antibodies, the diagnosis of immune thrombocytopenia still remains a clinical challenge (Fabris et al, 2004).The present study was designed to evaluate flow cytometry in the detection of platelet antibodies in comparison with MAIPA in a variety of immune thrombocytopenia. To achieve this aim a total of 90 subjects were enrolled. They were categorized into 18 ITP cases, 5 healthy controls and 50 patients with chronic HCV hepatitis, 12 cases with platelet transfusion refractoriness and 5 patients without platelet transfusion refractoriness served as control. All subjects were subjected to FCM measurement of PAIgs, FCM measurement of HLA Class I PRA and Measurement of antiplatelet antibodies by MAIPA. We found a significant elevation of PAIgs in patients with ITP compared to controls, There is significant elevation of HLA antibodies when compared with total PAIgs in platelet transfusion refractoriness patient. We conclude that PAIgs play a major role in the pathogenesis of ITP and their levels correlate with the type and activity of the disease. Immune based mechanism could contribute to thrombocytopenia in chronic HCV infection. Anti HLA antibodies are more associated with Platelet transfusion refractoriness. We recommend FCM could be used in screening of suspected immune thrombocytopenia and should be followed by MAIPA in positive cases to enhance the specificity of the assay.