الفهرس 
ImmunoglobulinsOnly 14 pages are availabe for public view
 |  | from | 141 |  |  |
| | | from | 141 | | |
Abstract
Toxoplasma gondii is an obligate intracellular parasite. Infection with this parasite caused toxoplasmosis, which consider one of the most common parasitic diseases. Toxoplasmosis can infect pregnant women, children, and immunosuppresed patients. Cats, rodents, meat, soil, water and other sources are sources of infection. Control of toxoplasmosis is done by education, vaccination and early diagnosis. In the present study five mice were injected with T.gondii of RH strain inorder to maintain Toxoplasma gondii tachyzoites, which used as a source of purified Toxoplasma antigen. The harvested tachyzoites were analyzed using SDSPAGE. Coomassie blue stains the separated polypeptides from 180KDa to 20KDa. The specific antiToxoplasma antibody was used as a probe in western blot for detection of target antigen. The lowest molecular weight sharp band (36KDa) is selected for study. 36KDa antigen was isolated from resolved tachyzoites crude antigen using electroelution from preparative gel which is then used in ELISA. Serum samples were collected from 92 pregnant women tested with IHA as a gold standard test. Standardization of ELISA technique showed that the optimum concentration of purified Toxoplasma antigen of maximum difference between the mean optical densities of infected and noninfected women is (50g/ml). The optimum dilution of serum sample showing maximum difference between the mean optical densities of infected and noninfected women is (1:100) and cutoff value (0.251). Serum samples were screened for antiToxoplasma IgM antibodies using ELISA, there were 19 out of 92 samples (21%) were negative while 73 out of 92 samples were positive for antiToxoplasma IgM antibodies(+veIgM) . only 60 samples out of 92 samples were (+veIgM) by IHA. This study ends to that ELISAIgM is more sensitive method than IHA in detction of recent Toxoplasma hnfection.