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Abstract Introduction: Pseudomonas aeruginosa is an opportunistic pathogen having the ability to infect a broad range of host organisms (He et al., 2004). It was known that P. aeruginosa does not possess the ability to hydrolyze esculin (Koneman et al., 1997a), but between (19981999), strains of P. aeruginosa hydrolyzing esculin were isolated for the first time in Russia (Sivolodskii, 2000a). Aim of Work: 1) Isolation and identification of esculinpositive P. aeruginosa biovar causing different infections in MUHs. 2) Finding out the incidence of esculinpositive P. aeruginosa biovar in MUHs. 3) Determination of antibiotic susceptibility pattern of this new strain. 4) Usage of esculin hydrolysis test as a typing method for tracing the source of infection in pseudomonal nosocomial outbreaks. Subjects & Methods: Samples were collected from different departments of Mansoura University Hospitals (MUHs) including both inpatients wards and outpatients clinics. Processing of the samples was done at the Microbiology Diagnostic and Infection Control Unit (MDICU) in the department of Medical Microbiology and Immunology. The isolated colonies were picked up and identified in a systemic manner for selection of esculinpositive P. aeruginosa biovar. Small scale preparation of plasmid DNA ?Miniprep? was performed to check the plasmid presence and the plasmid profiles of the 7 isolated esculinpositive P. aeruginosa and 7 esculinnegative strains. Results:1) The highest incidences of P. aeruginosa were found in burn wound infections, ear discharge, and pleural fluid specimens. The lowest incidences were observed in blood, stool, and throat swabs. 2) Esculinpositive P. aeruginosa biovar represented 3.3% of all isolated P. aeruginosa strains. 3) Esculinpositive P. aeruginosa biovar was isolated from SSI (57.1%), urine (28.6%), and eye swab (14.3%). |