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Abstract Fungal biosynthesis of silver nanoparticles (SNPs) offers a safe, eco-friendly and promising alternative technique for large-scale manufacturing. In this study, the ability of endophytic fungi was investigated to synthesize SNPs. Aspergillus flavipes AUMC 15772, a novel endophytic fungi isolated from the leaves of the Lycium shawii was able to synthesize SNPs extracellularly. One variable at a time (OVAT) and Plackett Burman design (PBD) were conducted for enhancing and optimizing the mycosynthesis of Aspergillus flavipes- silver nanoparticles (Af-SNPs). Comprehensive characterization using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and selected-Area Electron Diffraction (SAED) patterns confirmed the precise composition. |