Search In this Thesis
   Search In this Thesis  
العنوان
Bacteriological and molecular studies on methicillin resistance
Staphylococcus aureus isolated from meat products
المؤلف
Kozaa،Shireen Mohamed.
هيئة الاعداد
باحث / Shireen Mohamed Kozaa
مشرف / Ashraf Awad Abd El –Tawab
مشرف / Ahmed Saber ELFayoumy
مشرف / Marwa Awad Meawad Mohamed
الموضوع
Methicillin resistance. meat products .
تاريخ النشر
2024.
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
11/4/2024
مكان الإجازة
جامعة المنوفية - مكتبه كلية الطب البيطرى - البكتريا والفطريات والمناعة
الفهرس
Only 14 pages are availabe for public view

from 67

from 67

Abstract

S. aureus is among the most important bacteria causing food poisoning.
The contamination of food with different food borne pathogens constitutes
serious problems for consumers; therefore, the following study was
performed on 100 random samples of meat products, (burger, chicken
nuggets, luncheon and kofta.)
(25 for each) were collected from different shops and supermarkets at
Gharbia Governorate. In addition, studying the phenotypic characterization
of the isolated bacterial strain; in-vitro anti-microbial sensitivity for them
and genotypic characterization and detection of some virulence and
resistance genes of some isolates of S.aureus by PCR technique The results
of S. aureus isolation , revealed that, a total of 25 S.aureus isolates were
obtained.
S. aureus isolation showed that 25/100 (25 % strain were isolated 9/25
(36%) from burger, 7/25 (28%) from chicken nuggets, 6/25(24 %) from
luncheon, 3/25 (12%) from kofta.
The in- vitro sensitivity test for 25 isolates of S.aureus (revealed that, the
isolated S.aureus were highly resistant for penicillin and flucloxacillin
(96%) respectively , cefotaxime( 68%) , clindamycin (60 %) ,
erythromycin ( 56 % ), linezolid (36% )and vancomycin( 32 %).
The examined isolates of S.aureus showed the presence of some resistance
gene blaZ and mecA, the blaZ gene was amplified in only 3 isolates 3/6,
while mecA gene was detected in all examined isolates 6/6 and the virulence
genes (hlg) was detected in only 3 isolates 3/6 in all examined isolates .