الفهرس | Only 14 pages are availabe for public view |
Abstract Aim of study: Derivatization and determination of drugs with terminal alkyne group in pharmaceutical preparations and biological fluids based on click reaction. Methods: Spectrofluorimetric method and High-Performance Liquid chromatography with fluorescence detection. Results: • Spectrofluorimetric quantification of Ethinylestradiol in spiked human plasma after reaction with 4-azido-7-nitrobenzofurazan. • Preliminary pharmacokinetic study of Ethinylestradiol in rats by High-Performance Liquid chromatography with fluorescence detection. • Spectrofluorimetric determination of Levonorgestrel in breast milk after derivatization with 4-azido-7-nitrobenzofurazan. • Spectrofluorimetric determination of Rasagiline mesylate in human plasma after derivatization with 4-azido-7- nitrobenzofurazan. Conclusion: All the proposed methods have been developed, optimized and validated. Key words : Click reaction; 4-azido-7-nitrobenzofurazan; Fluorescence |