الفهرس | Only 14 pages are availabe for public view |
Abstract Summary: The present in vitro study was aimed to compare between the ability of three fruit vinegars ( apple, dates and pomegranate) and NaOCl followed by EDTA as root canal irrigants to inhibit the growth of E. faecalis biofilm in the root canal by counting the CFU/ ml and also their ability to remove intracanal smear layer by SEM. In the present study, 67 unidentified single rooted human premolars were divided into 5 equal groups (n=13): apple vinegar group, dates vinegar group, pomegranate vinegar group, NaOCl/ EDTA group and saline (control) group. The remaining 2 teeth were only used for confirmation of biofilm formation. Teeth were decoronated to standardize the root length, then all teeth were instrumented using Denjoy rotary files and irrigated only with saline solution between each file. All roots were sterilized then infected with clinical strain of E. faecalis and incubated for 21 days to allow mature biofilm formation. Initial microbial samples S1 were taken from the root canals with paper point technique then disinfection of root canals was carried out by irrigation with the corresponding irrigant. Second microbial samples S2 were taken immediately after the irrigation procedures. Then the roots were again incubated for 1 week after which the third microbial samples S3 were taken. For evaluation of smear layer removal, all roots were longitudinally sectioned and scanned with SEM at coronal, middle and apical thirds. Image analysis was carried out using imageJ software. Summary 88 Results of the present study showed that dates vinegar has the highest antibacterial action after 20 min of irrigation followed by NaOCl/EDTA with no statistically significant difference between the two groups. However, after 1 week, NaOCl/EDTA achieved the highest antibacterial action followed by dates vinegar with no significant difference. For smear layer removal , NaOCl/EDTA showed the highest ability to remove smear layer followed by dates vinegar with no significant difference. The antibacterial action of pomegranate vinegar was higher than apple vinegar after 20 min of irrigation with no significant difference. However, after one week, the antibacterial action of pomegranate was significantly higher than apple vinegar. The smear layer removal ability of pomegranate vinegar was non significantly higher than apple vinegar. Saline has significantly the least antibacterial action and smear layer removal ability. |