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Abstract This study was performed on 55 patients with hematologic malignancies (37 male and 18 female). Patients were subdivided into groups: patients with AML, patients with T-ALL, patients with B-ALL, patients with CLL, patients with lymphoma and patients with multiple myeloma. The following investigations were done 1. Full clinical evaluation including Entire history taking including: (age, sex, therapeutic history, blood transfusion).Clinical examination including: (anemic manifestation, fever, hepatomegaly, splenomegaly, lymphadenopathy, bleeding tendency, and bone tenderness) 2- Laboratory investigations include complete blood count: Two ml of venous blood were withdrawn into ethylene diamine tetra acetic acid (EDTA) used immediately for complete blood counting with spreading of peripheral blood smears for Leishman staining and differential leucocytic counting. CBC was done using ADVIA 2120i (Siemens, Germany), including hemoglobin (Hb) level, RBCs indices, total leukocytic count, differential leukocytic count, platelet count, platelet indices, and, reticulocytes. Examination of peripheral blood smears stained with Leishman stain for differential leukocytic count and detection of abnormal cells BMA: sample was collected in EDTA tube for each patient from the anterior or posterior superior iliac spine, half ml of BM aspirate was mixed immediately on glass slides, and smears were spread to be examined after staining by Leishman stain. One ml of BM aspirate was gently dispensed into an EDTA solution tube for Flow cytometry (FCM) immunophenotypic analysis. The sample used for FCM was evaluated. |