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Abstract Background: The present clinical study was conducted to assess the effect of application of gaseous ozone to palatal donor sites after harvesting of free gingival grafts (FGG) on pain perception and wound healing and epithelization compared to sites protected by periodontal dressing. Methodology: The study included twenty patients presenting twenty mucogingival defects that required augmentation with FGGs. The patients were randomized into 2 groups; the test group where the palatal donor sites were treated with ozone application and the control group where the palatal donor sites were protected by periodontal dressing after harvesting of the FGG. Postoperative pain was evaluated using VAS at the day of surgical intervention then, after 3,7,14, 21 and 28 days. In addition, the patients were asked to report the number of analgesic tablets ingested to relieve pain. Palatal wound healing was evaluated using Landry et al., (1988) as well as wound epithelialization test at 3,7,14, 21 and 28 days after surgical intervention. Results: There was an insignificant difference in VAS scores between control and test groups on Day 0, Day 14, 21, and day 28. The test group showed significantly lower VAS scores on day 3(p˂0.05) and day 7 (p˂0.01) compared to the control group. There was no statistically significant difference between test and control groups in % of sites that showed complete epithelialization on 7, 21 and 28 days. There was statistically significant difference at 14 days in favour of the test group ((p˂0.001). The test group exhibited significantly higher scores for the Landry et al., 1988 healing index at 7 ((p˂0.001) and 14 (p˂0.01) days compared to the control group. Conclusion: The application of gaseous ozone to palatal donor sites accelerated the wound healing process and reduced |