الفهرس 
Effect on resident T cells of the skinOnly 14 pages are availabe for public view
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Abstract
Psoriasis is a common chronic inflammatory disease of the skin affecting 1–2 % of the global population It is an immune-mediated disease mediated by the cells and molecules of innate and adaptive immune systems
The exact cause of psoriasis is unknown, but it may be the net result of the interaction among genetic, environmental and immunological factors Immunological factor of psoriasis pathogenesis is that it’s T cell –dependent autoimmune disease of the skin and joint. Disease manifestations is started by proinflammatory CD-4 positive T helper cells producing either interferon – gamma from T helper 1 cells or interleukin 17 from T helper 17 cells. These Th1 cells and Th17 cells interact with dermal dendritic cells, macrophages, mast cells, and neutrophils which can cause chronic skin inflammation of psoriasis Programmed cell death protein 1, also known as PD1 and CD279 (cluster of differentiation 279), is a protein on the surface of cells that has a role in regulating the immune system response to Th cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T- cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells
This study aims to evaluate & analyse the role of PD1 +ve TFH cells in psoriatic patients and its relation to the clinical signs.
The study involved 30 patients with psoriasis from Dermatology outpatient clinic, Menoufia university hospital and 15 apparently healthy volunteers as control group during the period between augustt 2022 and November 2022
A written consent form had been taken from patients and controls according to human rights and ethical committee, Faculty of Medicine- Menoufia University.
The study had been approved by the local ethics committee on research involving human subjects of Menoufia faculty of Medicine.
The study procedures were free from any harmful effects on the participants as well as the service provided.
The principal investigators have kept individual data as private information safely. There was no extra fee to be paid by the participants and the investigators covered all the costs in this regard.
Detection of PD-1 positive blood follicular helper T cells by immunophenotyping using CD4, CXCR5 and PD1 monoclonal antibodies using cytoflex.
5ml of blood was withdrawn by venipuncture from each subject and was divided into 2 EDTA tubes : the 1st for CBC and 2nd for IPT . The current study displayed that There is statistically significant increase in CD4 and CD4/CD249 in patients with psoriasis than healthy controls. While there is statistically significant decrease in CD185/CD249 and PD1in patients with psoriasis than healthy controls. There is statistically significant negative correlation between CD4, CD4/CD249 and PD1 and statistically significant positive correlation between CD185/ CD249 and PD1 among our studied patients.