الفهرس Only 14 pages are availabe for public view
 |  | from | 152 |  |  |
| | | from | 152 | | |
Abstract
Discovered as the top glucose-induced gene, thioredoxin - interacting protein (TXNIP) has now emerged as such a key player in pancreatic Ý - cell biology. Pancreatic Ý - cell expression of TXNIP gene has been found to be tightly regulated by multiple factors and to be dramatically increased in diabetic islets. Hyperglycemia and diabetes mellitus upregulate Ý - cell TXNIP gene expression, and TXNIP overexpression plays a critical role in the subsequent beta cell glucotoxicity and induction of pancreatic Ý - cell apoptosis. This study aims to evaluate the effect of verapamil, in different doses, on blood glucose and insulin levels as well as TXNIP gene expression in the Ý - cells of the pancreatic islets in STZ - induced diabetes mellitus in rats, shedding light on the mechanism involved. Diabetes was induced by a single intraperitoneal injection of freshly dissolved streptozotocin (STZ) 60 mg / kg body weight in 0.1 M sodium citrate buffer, pH = 4.5, to 18 h fasted 60 male adult albino sprague - dawley rats. Diabetic rats received oral verapamil at variable doses (7 and 14mg / kg / day) and mixed insulin 16 unit / kg / day subcutaneous injection; both once daily for four weeks