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Abstract
Strain tolerance to toxic metabolites remains an important issue in the production of biofuels. The impact of overexpressing the heterologous groESL chaperone from Clostridium acetobutylicum to enhance the tolerance of Escherichia coli against several stressors were examined. Strain tolerance was identified using strain maximum specific growth rate (æ) and strain growth after a period of solvent exposure. In comparison with control strain, the groESL overexpressing strain yielded a 27% increase in growth under 0.8% (v/v) butanol, and a 64% increase under 1.75 (g/l) acetate. Moreover, after 10 hours, groESL overexpression resulted in increase in relative tolerance of 58% compared with control strain under 0.8% (v/v) butanol, 42% increase under 1% (v/v) isobutanol, 36% increase under 4% (v/v) ethanol, and 58% increase under 1.75 (g/l) acetate. These data demonstrate that overexpression of the groESL from Clostridium acetobutylicum in Escherichia coli increased tolerance to several stressors