الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
This study was planned to exploit the most recently innovated noble nanoparticles in a highly sensitive colorimetric real-time-assay for detecting E. coli O157:H7 in feedstuffs. Gold nanoparticles (AuNPs) were prepared adopting the citrate method and the obtained colloidal nanoparticle suspension was characterized using the UV{u2013}vis spectroscopy, Dynamic light scattering (DLS), Zeta potential instrument, Transmission Electron Microscopy (TEM) and X{u2019}Pert PRO X-ray Diffraction System (XRD). Results showed that the prepared AuNPs were well dispersed colloidal spherical shaped nanosized particles averaged 40 nm in size having a characteristic surface plasmon resonance band similar to gold nanoparticles and absorbance peak of 530 nm. Zeta potential of the prepared AuNPs verified the negatively charged surfaces of -32 mv. Specific E. coli O157:H7 antiserum was conjugated to the surfaces of the prepared AuNPs and the developed AuNPs-conjugated suspension had a red color. Stability of the AuNPs- E. coli O157:H7 antiserum conjugate suspension was found to be greatly affected by the antibody concentration used in the conjugation mixture.