الفهرس 
Title : Assessment of some placental non-coding RNAs as serum biomarkers for preeclampsia
AbstractOnly 14 pages are availabe for public view
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Abstract
New predictors that could boost early detection of preeclampsia (PE) and prognosticate its severity are urgently needed. This study examined serum miR-17, miR-363, MALAT-1 and HOTAIR as potential biomarkers of PE risk, onset and severity in Egyptian women with PE. This prospective study included 160 pregnant females; 82 PE cases and 78 healthy pregnancies. Serum samples were collected between 20 to 40 weeks of gestation. Early-onset PE (EOPE) was defined as developing clinical manifestations at ≤ 34 gestational weeks. Severe PE was defined as systolic blood pressure (SBP) ≥160 mmHg and/or diastolic blood pressure (DBP) ≥110 mmHg and proteinuria (≥2 g/24 hours or ≥2+ dipstick). selection of PE-related non-coding RNAs (ncRNAs) and functional target gene analysis were conducted via bioinformatics analysis. Expression profiles were assessed by RT-qPCR. Serum miR-363 and MALAT-1 were downregulated, meanwhile miR-17 was upregulated, and HOTAIR was not significantly altered in PE patients compared with healthy pregnancies. MiR-17 was elevated while miR-363 and MALAT-1 were reduced in severe versus mild PE. MiR-363 was lower in EOPE versus late-onset PE (LOPE). MALAT-1, miR-17 and miR-363 showed diagnostic and discriminated severe PE, whereas miR-363 distinguished EOPE in the receiver-operating-characteristic (ROC) analysis. MiR-363 and MALAT-1 were significantly associated with early and severe PE, respectively in multivariate logistic analysis. In PE, miR-17 and MALAT-1 correlated with gestational age (GA) (r= -0.328 and r= 0.322, respectively) and albuminuria (r= 0.312 and r= -0.35 respectively). In this study, the MALAT-1, miR-363 and miR-17-related protein-protein interaction (PPI) networks linked to PE were constructed. Serum miR-17, miR-363 and MALAT-1 could have utility as new biomarkers of PE diagnosis. MiR-363 may be associated with EOPE andMALAT-1 downregulation correlates with PE severity.