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Abstract Two field experiments were carried out in the two seasons 2019/2020 and 2020/2021 to evaluate the effect of Abscisic acid and proline on the morpho-physiological features, and yield component of two wheat cultivars (Sids14 and Giza171) under water deficit stress (moderate drought and severe drought). Our results displayed that number of tillers, leaf area, spike length (cm), relative growth rate, spike length (cm), plant height (cm), number of grains/spike, 1000 grains weight (g), grain yield (ton/ha), harvest index (%) negatively affected under both moderate and severe drought in the two cultivars. Moreover, chlorophyll a, chlorophyll b concentration and relative water content were significantly reduced under drought conditions in the two seasons.Application of Abscisic acid and proline led to improve morphological characters such as spike length (cm), relative growth rate, spike length (cm), plant height (cm), number of grains/spike, 1000 grains weight (g), grain yield (ton/ha), harvest index (%) in the drought stressed plants compared with untreated drought stressed plants. Additionally, the treatment with Abscisic acid and proline under drought conditions led to improve chlorophyll a, chlorophyll b concentration and relative water content in the stressed wheat plants compared with untreated stressed plants in both seasons. It can conclude that, application of Abscisic acid and proline can ameliorate the negative effects of water deficit on wheat cultivars Sids14 and Giza171 and improve grain yield. Key wards: wheat, Abscisic acid, proline, water deficit, chlorophyll, relative water content, yield. |