الفهرس 
CerebellumOnly 14 pages are availabe for public view
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Abstract
Monosodium glutamate is one of the most commonly used flavorenhancing substances that may lead to neurological disorders. The aim of the present work was to study the possible role of ALA on the MSGinduced motor coordination deficits in male albino rats when used as pretreatment, cotreatment and post-treatment to MSG. This was carried out by assessment of motor coordination using Rotarod, triple horizontal bars, static rods tests and footprint analysis. To investigate the possible mechanisms behind the actions of ALA, serum oxidative stress markers (MDA and TAC) and inflammatory marker (TNF-α) and also cerebellar BDNF and Oxidative DNA damage were measured. Furthermore, H&E staining of the cerebellum, number of purkinjie cells and molecular layer length were assessed. Seventy male albino rats were used (age 2-3 months, each weighing 160-200g). The rats were randomized into the following groups: Control (C) group, (2) Non-treated monosodium glutamate administered (MSG) group, (3) Alpha-lipoic acid pretreated monosodium glutamateadministered (ALA pretreated) group, (4) Alpha-Lipoic acid cotreated monosodium glutamate-administered (ALA cotreated) group, (5) AlphaLipoic acid-post-treated monosodium glutamate-administered (ALA posttreated) group. At the end of the experimental time, motor coordination was assessed in animals of all groups using Rotarod, Triple horizontal bars, Static rods tests and Foot print analysis. The serum oxidative stress biomarkers, including MDA and TAC, and inflammatory marker (TNF-α) and also cerebellar BDNF and Oxidative DNA damage levels were measured. Also, H&E staining of the cerebellum, number of purkinjie cells and molecular layer length were assessed.
The results showed that MSG caused significant deterioration in motor coordination, evidenced by an increase in the number of falls and a decrease in the latency period on the running surface of rotarod apparatus, a decrease in the score of triple horizontal bars test, an increase in orientation and transit times of static rods test, a decrease in Stride length and an increase in Stride Width in MSG 3 wks and MSG 6 wks groups when compared with control group. A significant increase in serum MDA level, significant decrease in serum TAC and significant increase in serum TNF- alpha and also significant declines in BDNF and elevation in Oxidative DNA damage in cerebellar tissue homogenate were also observed, denoting oxidative stress induction by MSG. The H&E staining showed significant decrease in the total number of purkinjie cells and significant decline in molecular layer length were also observed in MSG administered groups.