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العنوان
The Role of E93 gene in reproduction of female
red flour beetle, Tribolium castaneum :
المؤلف
Duaa Musleh Ibrahim Soliman Eid,
هيئة الاعداد
باحث / Duaa Musleh Ibrahim Soliman Eid,
مشرف / Afaf Abdel Meguid Abdel Wahab
مشرف / Hesham Ahmad Yousef
مشرف / Azza Mohamed Elgendy
تاريخ النشر
2022.
عدد الصفحات
80 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم الحشرات
تاريخ الإجازة
18/6/2022
مكان الإجازة
جامعة القاهرة - كلية العلوم - Entomology
الفهرس
Only 14 pages are availabe for public view

from 149

from 149

Abstract

The E93 transcription factor is a member of helix‐turnhelix
transcription factor family containing a Pip‐squeak
motif. This ecdysone primary response gene was identified
as a regulator of cell death in Drosophila melanogaster
where it is involved in ecdysone‐induced autophagy and
caspase activity that mediate degeneration of larval tissues
during metamorphosis from larva to pupa. However,
its function in adult insects is not well studied. To study
E93 function in the red flour beetle, Tribolium castaneum,
double‐stranded RNA (dsRNA) targeting E93 (dsE93) was
injected into newly emerged adults. Knockdown of E93
caused a decrease in the synthesis of vitellogenin (Vg),
oocyte development, and egg‐laying. Sequencing of RNA
isolated from adults injected with dsE93 and control
dsmalE (dsRNA targeting Escherichia coli malE gene) followed
by differential gene expression analysis showed
upregulation of genes involved in the metabolism of reserved
nutrients