الفهرس 
Stem CellsOnly 14 pages are availabe for public view
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Abstract
UC is a chronic inflammatory condition that causes continuous mucosal inflammation of the colon, usually without granulomas on biopsy. It affects the rectum and to a variable extent the colon in a continuous fashion, and is characterised by relapsing and remisson course
MSCs can be easily isolated and accelerated from a variety of tissues, including bone marrow-derived stem cells, adipose tissue-derived stem cells, umbilical cord stem cells,endometrial regenerative cells, placenta, intestinal stem cells, amnion amniotic fluid and tonsil.
MSCs exert protective functions and support the survival and regeneration of colonic epithelial cells and mucous barriers through the production of exosomes, growth factors, cytokines, and metabolites
This study aimed to investigate the ability of MSCs to differentiate into enterocytes and study the effect of differentiated MSCs in the treatment of acetic acid-induced ulcerative colitis in a mouse model.
MSCs were isolated from the umbilical cord and induced to differentiate into enterocytes. Induced cells were evaluated by morphological changes, flow-cytometric, and immune cytochemical analysis. Forty rats were divided into four groups: control, acetic acid induced UC, differentiated and undifferentiated treated groups. The acute colitis in rats was induced by administering 2ml of 3% AA transrectally. Body weight changes, disease activity index (DAI), histo pathological and immune histo chemical study for CD105 and CD34 were recorded.
In this study MSCs were cultured on DMEM supplmented with CBS as it is rich in growth factors needed for the cells and free of zoonotic pathogens and proteins.
MSCs were cultured on low glucose DMEM was used and this was associated with good isolation of MSCs.
MSCs cell exhibited fibroblast-like morphology after ten days. These cells gradually reached 50%-60% confluence at about 14days.
By flow cytometry, MSCs showed positive expression for CD44, and CD73 and negative expression for CD34.
Activin A was used for 3 days then FGF2 was added to promote the differentiation of DE into ISC-like cells.
Differentiated ISC-like cells were confirmed by morphology which appears as enlarged spiky-shaped cells with a rounded central nucleus.
The cells were positive for Musashi-1 immunostaining in which cell characteristics appear clearly.