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العنوان
Monitoring Of Bacillus Species In Milk And Some Dairy Products /
المؤلف
Morsy, Bahaa Abdel-Fattah Mohamed.
هيئة الاعداد
باحث / بهاء عبد الفتاح محمد مرسي
مشرف / عادل مصطفي الخولي
مشرف / عرفة مشرف سليمان
الموضوع
Dairy products. Dairying.
تاريخ النشر
2023.
عدد الصفحات
93 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علم الحيوان والطب البيطري
الناشر
تاريخ الإجازة
6/5/2023
مكان الإجازة
جامعة بني سويف - كلية الطب البيطرى - الرقابة الصحية على الالبان ومنتجاتها
الفهرس
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Abstract

This work was designed to detect bacteriologically the presence of Bacillus cereus in heat treated milk and some dairy products by using HiCrome™ Bacillus Agar and evaluating the significance of added cinnamon on growth of Bacillus cereus in prepared rice pudding.
A total of two hundred samples including UHT milk, pasteurized milk, rice pudding and infant food (50 of each) were collected randomly from different dairy shops, pastry shops and supermarkets at different localities in El-Fayoum city, Egypt. The obtained result showed that:
1- The mean values of B. cereus count in the examined samples of UHT milk, pasteurized milk, rice pudding and infant food were 5.9 × 102 ± 3.3 × 102 ,1.2 × 105 ± 5.7 × 104, 2.1 × 105 ± 7.4 × 104 and 4.1 × 103 ± 7.9 × 102 (cfu / ml or g) , respectively.
2- Other Bacillus spp. could be detected in the examined samples including B. thuringiensis, B. subtilis, B. megaterium, B. coagulans and B. pumilis .The incidence in UHT milk was 4 (8%) , 3 (6%), 0 (0%) , 3 (6%) and 0 (0%), pasteurized milk was 8 (16%) , 5 (10%), 2 (4%) , 4 (8%) and 0 (0%), rice pudding was 12 (24%) , 3
(6%), 2 (4%) , 5 (10%) and 2 (4%), while infant food was 11 (22%) , 4 (8%), 4 (8%) , 0 (0%) and 3 (6%), respectively.
3- Ten B. cereus isolates were checked for the presence of the hemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), cytotoxin K (CytK) and cereulide (ces) as virulence factors of B. cereus. Diarrheal toxins Hbl, Nhe and CytK could be located in 60, 100 and 100%, while emetic toxin ces could be located in 40% of the total B. cereus isolates.
4- Addition of cinnamon powder to rice pudding significantly affects the viability of B. cereus in examined samples ( p < 0.05), as the reduction % in count of B. cereus at 1 % cinnamon concentrations after 12, 24, 36 and 48 h. were 40, 91.7, 99.3, 98.9 %, respectively.
The significance and public health hazards of the isolated organism as well as suggestive control measures for improving the sanitary and microbiological conditions of such dairy products were discussed.