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Abstract
This study was carried out aiming to study morphological and growth behavior of produced calli of Salix safsaf Forssk plant and estimating their morphogenic capacities via organogenesis ”indirect and direct regeneration”. Moreover, investigate the active ingredient Salicin content (g/100gDW) produced in shoots either in vivo or/and in vitro in both developed calli and regenerated shoots. The most common outcomes proved that; survivals percentages varied between 0 to 99 % as both Clorox concentration and soaking period changed. Immersion explants in 30% Clorox solution containing 3-5 drops of Tween 20 for 20min and followed by three times rinses in sterile distilled water showed no contaminated explants