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Abstract
In this study a total number of 300 milk samples (100 from buffaloes, 150 from cows and 50 from pasteurized milk) were collected from different farms and supermarkets, samples from buffaloes were identified as 28 normal milk samples, 2 subclinically mastitic milk samples and 70 clinically mastitic milk samples, while the total 150 cow{u2019}s milk samples were identified as 23 normal milk samples, 17 subclinically mastitic milk samples and 110 clinically mastitic milk samples. the total bacillusspecies isolates were 48 (16%) from 300 milk samples (13 from buffaloes, 25 from cows and 10 from pasteurized milk) 6.25% from the total isolates were originated from subclinically mastitic animals, 14.6% from healthy animals, 58.3% from clinically mastitic animals and 20.8% from pasteurized milk samples.Bacterial identification indicated that Bacilluscereus constituted the higher percentage 45.8% (22 isolate), B.badiusisolates constituted 12.5 % from the total isolated species, and B.mycoides 10.4%, while species were identified asP.macerans, B.lechniformis, S.globisporas, M.marinus, B.subtilis and B.lentus in a percentage of 4.7%, 4.7%, 4.7%, 6.25%, 6.25%, and 6.25% respectively. Bacillus spp. in milk are generally sensitive to the antimicrobials commonly used for treatment specially chloramphenicol with high percentage of sensitivity (47.92%). Taking in consideration the exception of the high resistance to amoxycilin recorded in this study (83.3%). Results of virulence assay showed that most of the 48 Bacilli isolateswere positive for Congo red, biofilm and hemolytic activity in a percentage of 79.17%, 85.4% and 75% respectively. The verocell (cytopathic effect) of 48 isolates of Bacillusspecies recovered from cow, buffalo and pasteurized milk samples on MDCK cells were 100% in B.cereus, B.mycoides, B.badius, M. marinus, p.macerance, B.subtilis, B. lentus, B.lechniformis and S.globiosporus. Using PCR technique for Bacilli, a quick readings for the genotypic test showing that the highest readings is according ”BceT” toxin.It shows positive readings with 32 strains from 48 which is representing 67 % of strains. The lowest positive readings were come from two toxins ”EntFM” and ”EM” representing 41.7% each