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Abstract
A total of 176 samples (25 sample of broiler chicken, 25 samples of 1 day old imported chicks, 60 samples of imported frozen chicken, 15 samples of chicken fillet, 15 samples of chicken nuggets, 12 samples of chicken shawarma, 12 samples of chicken luncheon and 12 samples of chicken stock cubes) examined bacteriologically for coagulase negative staphylococci (CNS), 40 samples were positive for coagulase negative staphylococci with a total prevalence of 22.7%, while 34.6% were coagulase positive S. aureus. CNS revealed from 20% of broiler chickens, 12 % of one day old chicks, 15% of imported frozen chickens, 33.3% of chicken fillet, 46.6 % of chicken nuggets, 33.3 % of chicken shawarma, 50 % of chicken luncheon and 8.3% of chicken stock cubes, identification ocuured by 3 methods 1- biochemiclly by catalase test , oxidase test and coagulase test, 2- by PCR using 16S rRNA and clfa primers, 3- Integral system stafilococchi kit was used for identification of 40 isolates of CNS which indicated that 15 isolates were S. xylosus (with the highest percentage 37.5% ), 7 isolates were S. warneri (17.5%), 6 isolates were S.saprophyticus (15%), 3 isolates were S. hominis (7.5%), 4 isolates were S. gallinarum (10%), 3 isolates were S. simulans (7.5%) and 2 isolates were S. sciuri (5%). CNS strains were tested for antimicrobial sensitivity using Integral system stafilococchi kit and the higher sensitivity was showed to gentamicin, levofloxacin and claritromycin, while higher resistance was showed to amoxicillin + clavulanic acid