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Abstract
A total of 100 samples of (70 dog blood samples, 15 human blood samples, 15 hard ticks) were used to study the prevalence of Borrelia burgdorferi . Blood films were examined for at least 100 oil immersion fields (OLFs), for detection of helically shaped Borellia burgdorferi. No spiral shape bacterium was found in any blood smear of the examined dog. Borrelia burgdorferi could not be recovered from all the given samples by culturing on BSK-H medium, while seroprevalence to B burgdorferi 23% in canine patients while seroprevalence in contact human were (20%)