الفهرس 
Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
Vitiligo is an acquired depigmenting muco-cutaneous disorder
characterized by melanocytes loss. Its universal incidence ranges from
0.5% to 2% of population without any racial or sexual differences.
Vitiliginous patches contain either reduced or absent melanin. The
patches are initially small but they progressively enlarge and coalesce
giving large patches. Many hypotheses have been supposed to explain
the mechanism of melanocytes destruction, including autoimmune
mechanisms, cytotoxic mechanisms, an intrinsic defect of
melanocytes,oxidant–antioxidant mechanisms, melanocytorrhagy and
neural mechanisms.
Cold-inducible RNA-binding protein (CIRP), also called
CIRBP or A18 hnRNP, it is a member of the cold shock protein
family. It is a glycine-rich RNA chaperone that facilitates RNA
translation.
Cold-inducible RNA-binding protein (CIRP) has been defined
as an inflammatory mediator and a damage-associated molecular
pattern (DAMP), which is highly expressed during trauma and shock.
It can induce a variety of cellular responses including the release of
proinflammatory cytokines and endothelial dysfunction.
The current study aimed to shed light on the possible role of
CIRP in the pathogenesis of vitiligo through evaluation of CIRP tissue
and serum levels, in addition to correlate the evaluated results with the
clinical aspects of vitiligo in those patients.
Recent studies reported that CIRP p‟ay its role through binding
to the TLR4/MD2 complex on both CD8+ and CD4+ T-cells to induce
Summary
91
their activation and stimulates the release of TNF-α and HMGB1 from
antigen-presenting cells.
This study aimed to evaluate the expression of CIRP, (serum
and tissue), in vitiligo cases and to correlate this expression to the
clinico-pathological data of the patients.
This case-control study included twenty patients presented with
vitiligo. They were selected from Outpatient Clinic, Dermatology,
Andrology and STDs Department, Faculty of Medicine, Menoufia
University and twenty age and sex matched healthy subjects as a
control group who attending Plastic Surgery OutpatientClinic,
Menoufia University.
Exclusion Criteria included dermatological diseases other than
vitiligo, history of malignancy, inflammatory diseases or symptoms of
any infections.
A written consent was obtained all patients were subjected to
complete history taking, dermatological examination and registration
of VAsIscore. 3 millileters of venous blood samples were taken under
complete aseptic conditions from every participant.The samples
centrifuged for 10 min at 4000 r.p.m.. The serum obtained for
assessment of serum CIRP by ELISA technique. Skin biopsies were
taken under local anaesthesia from all cases (lesions) and controls for
histopathological and immunohistochemical evaluation using H&E
and CIRP antibody stains.
In the current study, Positive expression of Cold inducible RNA
protein (CIRP) was observed in all cases (20 cases 100%) in lesional
and prelesional tissue in addition to control group (20 cases 100%)
with nuclear localization in all of the stained cells.
Summary
92
A significant difference in CIRP epidermal expression between
lesional, perilesional cases and controls was observed. It was high in
cases lesional and perilesional epidermis.
There was a highly significant difference between cases and
controls regarding mean serum cold inducible RNA binding protein
level, as it was higher in cases than controls (P value <0.001)
There was significant positive correlation between serum cold
inducible RNA protein and VASI score among the studied cases (P
value 0.013).
There was no significant relation between H score of lesional
epidermis and histo-pathological data of the studied cases was found.
No significant relation between serum cold inducible RNA
binding protein and clinical data of the studied cases except for family
history, cases with positive family history had higher serum level of
CIRP (318.2±48.0) than cases with negative history (254.0±20.6),
(p=0.005).So we recommend Further large scaled studies to validate
our results.