الفهرس 
Introduction and Aims of the studyOnly 14 pages are availabe for public view
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Abstract
Medicine plants have been an integral part of human society since the early beginning of
civilization for the treatment of diseases. In traditional literature such as that of ancient Egyptian
and Persian Aloe Vera’s therapeutic advantages and healing properties have been appreciated for
generations. The aim of this study was to determine the effect of Aloe Vera leaf extract on the
cells of Swiss albino mice. We focused on the mitotic index and chromosomal abnormalities in
mouse bone marrow cells. A complete blood count was also performed to detect any additional
effects of aloe leaf extracts. In cages made of polypropylene, 32 mice were kept. Throughout the
experiment, the animals were given a conventional diet, water at will, and bedding materials made
of wood sawdust and shavings. For four weeks, three groups received daily oral treatments using
gavage needles containing freeze-dried powdered alcoholic Aloe Vera extract in doses of 40, 80,
and 120 mg/kg in distilled water, respectively. Three additional groups received daily oral
treatments using the same dose plus 100 mg/kg of ARA-C (Tabine® 1000 mg/10 ml) administered
subcutaneously. One group served as a positive control and received daily oral treatments using
distilled water, while the other group served as a negative control.
The results showed the following:
1. The mitotic index was significantly reduced by Aloe Vera leaf extract to a level lower than
the negative control.
2. ARA-C reduced the mitotic index to about a third of the negative control.
3. The combination of ARA-C with different concentrations of Aloe Vera leaf extract
decreased the mitotic index at the concentrations of ARA-C + 40 mg/kg and ARA-C + 80
mg/kg Aloe Vera leaf extract. However, the concentration of ARA-C + 120 mg/kg Aloe
Vera leaf extract induced a significant increase in the mitotic index.
4. Aloe Vera leaf extract alone reduced chromosomal aberrations, but this reduction is not
dependent on the concentration of Aloe Vera leaf extract.
5. ARA-C induced a significant increase in chromosomal aberrations. ARA-C + 80 mg/kg
and 40 mg/kg Aloe Vera leaf extract reduced the chromosomal aberrations, and a great
reduction was observed with ARA-C + 120 mg/kg Aloe Vera leaf extract.
6. The highest frequency of Micronucleus was observed in mice treated with ARA-C,
followed by ARA-C + 120 mg/kg Aloe Vera leaf gel extracts and ARA-C + 40 mg/kg Aloe
Vera leaf gel extracts. Which indicates that Aloe Vera leaf gel extracts reduced the
micronucleus ratio to 1/10 of the ratio of ARA-C alone.
7. White blood cells were significantly increased by Aloe Vera extract, and it has the potential
to counteract the negative effects of ARA-C treatment but does not reach the negative
control level. And this effect is not dose-dependent.
8. Measuring Red blood cell count (RBC) shows a significant decrease from the negative
control in Aloe Vera 40 mg/kg and Aloe Vera 80 mg/kg. An insignificant decrease in Aloe
Vera 120 mg/kg. A significant increase from ARA-C treated mice in ARA-C + Aloe Vera
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40 mg/kg and ARA-C + Aloe Vera 120 mg/kg treated mice but in ARA-C + Aloe Vera 80
mg/kg showed an insignificant decrease.
9. A significant Hemoglobin level (HGB) increase is shown in ARA-C +Aloe Vera 120 mg/kg
over ARA-C, ARA-C + Aloe Vera 40 mg/kg and ARA-C + Aloe Vera 80 mg/kg treatments.
And no significant difference between Control, Aloe Vera 40 mg/kg, Aloe Vera 80 mg/kg
and Aloe Vera 120 mg/Kg.
10. A significant Lymphocytes count increase in Aloe Vera 80 mg/kg and Aloe Vera 120
mg/Kg than the negative Control. And The ARA-C +Aloe Vera 120 mg/kg treatment shows
a significant increase over ARA-C, ARA-C + Aloe Vera 40 mg/kg and ARA-C + Aloe
Vera 80 mg/kg.
11. Significant increases in uric acid levels appear in Aloe Vera 80 mg/kg, Aloe Vera 120
mg/kg, ARA-C, ARA-C + Aloe Vera 40 mg/kg, ARA-C + Aloe Vera 80 mg/kg, and ARAC + Aloe Vera 120 mg/kg more than the negative control. And significant decreases appear
in ARA-C + Aloe Vera 40 mg/kg, ARA-C + Aloe Vera 80 mg/kg, and ARA-C +Aloe Vera
120 mg/kg more than the ARA-C treatment.
The obtained result revealed that more research is required to properly comprehend the
active principles and the precise mechanism of action of Aloe Vera leaf extract because the
data that have been collected are insufficient to evaluate Aloe Vera components.