الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Periodontitis is a global disease that affects abroad age range. It can be caused by the interaction between periodontal pathogens and host immune response. Certain bacteria have a key role in pathogenesis of periodontal disease such as A. actinomycetemcomitans and P. gingivalis. These Gram-negative bacteria play an important role in the development and progression of periodontal disease, being major putative periodontal pathogens related to periodontitis (grade C), and periodontal therapy failure
The present study conducted to investigate the levels of A. actinomycetemcomitans and P. gingivalis in GCF after intra-pocket diode laser therapy as adjunct to non-surgical treatment in severe periodontitis (grade C). The present study included fifty patients having severe periodontitis (stage III grade C), PPD≥ 6, CAL ≥ 5, with age range from 15 to 35 years old, no history of taken any antibiotic therapy or mouthwash containing antimicrobials for the past three months. All subjects were evaluated clinically and radiographically at the first visit for diagnosis and selection of the patients. After selection subjects, GCF samples were collected after drying the area, PPD and CAL were assessed, and SRP was performed for each subject. These patients were divided equally into two groups: test group, was managed by intra-pocket diode laser therapy application and control group, was managed by antibiotic adminstartion. Patients were recalled for follow-up at one month and three months to collect GCF samples and reassess PPD and CAL. The GCF samples were sent to microbiological lab and analysed by Real-Time PCR.
The results of the study showed a non-statistically significant difference both in clinical and microbiological parameters between the two groups. However, comparing each group individually there was a statistically significant decrease concerning PPD and CAL more in control group than the test group. Concerning the levels of A.actinomycetemcomitans and P.gingivalis the significant decrease was more in the test group than the control one.