الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
 |  | from | 216 |  |  |
| | | from | 216 | | |
المستخلص
Hepatitis C virus (HCV) is the most common chronic blood-borne infection in the United States and some other parts of the world including Egypt, with the majority of patients becoming chronically infected and a subset (” " ~ " ”20%) progressing to cirrhosis and hepatocellular carcinoma. Individual variations in immune responses may help define successful resistance to infection with HCV. The present study aimed to identify the role of toll-like receptor 3 (TLR3).rs3775290 (c.1377C/T), TLR9.rs5743836 (-1237T{u2192}C) and TLR9.rs352140 (G2848A) gene polymorphisms to predict the outcome of HCV infection. We enrolled 404 healthcare workers (HCWs) and 142 patients in four groups: group 1 included 265 seronegative, aviremic subjects; group 2 included 25 seronegative, viremic subjects; group 3 included 87 subjects with spontaneously resolved HCV infection; and group 4 included 169 chronic HCV patients. All subjects were genotyped for TLR3.rs3775290 (c.1377C/T), TLR9.rs5743836 (-1237T{u2192}C) and TLR9.rs352140 (G2848A) single nucleotide polymorphisms (SNPs). We, also, quantified HCV-specific cell-mediated immunity (CMI) in 265 HCWs using an interferon gamma (IFN-Þ) enzyme-linked immunospot (ELISpot) assay in response to 9 HCV genotype 4a overlapping 15mer peptide pools covering the whole viral genome. TLR3 genotype CC (rs3775290) was associated with chronic HCV-infection where there was a greater frequency of this genotype among chronic subjects when compared to spontaneously resolved patients (63.9% versus 51.9%; { 2=6.81, p=0.033). No significant association was found between TLR9.rs5743836 (-1237T{u2192}C) and TLR9.rs352140 (G2848A) genotype, and allele frequency among subjects with chronic infection and spontaneous clearance of HCV